Coexpression and estrogen‐mediated regulation of TRPV6 and PMCA1 in the human endometrium during the menstrual cycle
Identifieur interne : 002578 ( Main/Exploration ); précédent : 002577; suivant : 002579Coexpression and estrogen‐mediated regulation of TRPV6 and PMCA1 in the human endometrium during the menstrual cycle
Auteurs : Hyun Yang [Corée du Sud] ; Kyung-Chul Choi [Corée du Sud] ; Sang-Hwan Hyun [Corée du Sud] ; Eui-Bae Jeung [Corée du Sud]Source :
- Molecular Reproduction and Development [ 1040-452X ] ; 2011-04.
English descriptors
- Teeft :
- Calcium homeostasis, Calcium ions, Embryo implantation, Endometrial, Endometrial epithelial cells, Endometrial tissues, Endometrial trpv6, Endometrium, Epithelial, Estrogen, Estrogen receptor, Estrous cycle, Experimental analyses, Fetal implantation, Gapdh, Glandular epithelial cells, Homeostasis, Human endometrial tissues, Human endometrium, Immunohistochemical analysis, Implantation, Ishikawa, Ishikawa cells, Jeung, Menstrual, Menstrual cycle, Menstrual phase, Mrna, Negative control, Physiol, Plasma membrane, Pmca1, Pmca1 expression, Pmca1 expression levels, Pmca1 mrna, Pmca1 mrna expression, Pmca1 mrna levels, Present study, Primer, Proc natl acad, Progesterone, Proliferative, Proliferative phase, Proliferative phases, Protein levels, Receptor, Reprod, Salamonsen, Santa cruz, Secretory, Secretory phase, Starvation media, Steroid, Steroid hormones, Transporter, Trpv6, Trpv6 mrna, Uterine, Uterine endometrium, Uterine trpv6, Uterus.
Abstract
Maintenance of calcium balance in the uterus is essential for many of its functions, including embryo implantation. The plasma membrane Ca2+‐pumping ATPase proteins are encoded by four genes designated PMCA1‐4, and PMCA1 is expressed in the uterus of rats during the estrous cycle. Although transient receptor potential cation channel subfamily V, member 6 (TRPV6), has been detected in the human placenta, pancreas and the prostate gland, expression patterns of uterine TRPV6 and PMCA1 and their potential roles in the human endometrium remain to be elucidated. In the present study, the expression patterns of TRPV6 and PMCA1 were examined to predict their potential roles in the human endometrium during the menstrual cycle. Human classified endometrial tissues (total n = 40) were separated into three groups according to menstrual cycle phase: menstrual, proliferative (early‐, mid‐, late), and secretory phase (early‐, mid‐, late). The expression of TRPV6 and PMCA1 mRNA and protein in the uterine endometrium during the menstrual cycle increased by 1.5‐ to 1.8‐fold at the proliferative phase (early‐, mid‐, and late‐) in comparison to the other phases. Estrogen treatment caused a significant increase in TRPV6 and PMCA1 mRNA expression. Immunohistochemical analysis of the distribution of TRPV6 and PMCA1 in the uterus revealed that both proteins are abundantly expressed in the cytoplasm of endometrial and glandular epithelial cells during menstrual phases. Taken together, these results suggest that uterine expression of TRPV6 and PMCA1 may be involved in human reproductive function. Mol. Reprod. Dev. 78:274–282, 2011. © 2011 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/mrd.21303
Affiliations:
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361‐763</wicri:regionArea><wicri:noRegion>361‐763</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Molecular Reproduction and Development</title><title level="j" type="alt">MOLECULAR REPRODUCTION AND DEVELOPMENT</title><idno type="ISSN">1040-452X</idno><idno type="eISSN">1098-2795</idno><imprint><biblScope unit="vol">78</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="274">274</biblScope><biblScope unit="page" to="282">282</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-04">2011-04</date></imprint><idno type="ISSN">1040-452X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1040-452X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Calcium homeostasis</term><term>Calcium ions</term><term>Embryo implantation</term><term>Endometrial</term><term>Endometrial epithelial cells</term><term>Endometrial tissues</term><term>Endometrial trpv6</term><term>Endometrium</term><term>Epithelial</term><term>Estrogen</term><term>Estrogen receptor</term><term>Estrous cycle</term><term>Experimental analyses</term><term>Fetal implantation</term><term>Gapdh</term><term>Glandular epithelial cells</term><term>Homeostasis</term><term>Human endometrial tissues</term><term>Human endometrium</term><term>Immunohistochemical analysis</term><term>Implantation</term><term>Ishikawa</term><term>Ishikawa cells</term><term>Jeung</term><term>Menstrual</term><term>Menstrual cycle</term><term>Menstrual phase</term><term>Mrna</term><term>Negative control</term><term>Physiol</term><term>Plasma membrane</term><term>Pmca1</term><term>Pmca1 expression</term><term>Pmca1 expression levels</term><term>Pmca1 mrna</term><term>Pmca1 mrna expression</term><term>Pmca1 mrna levels</term><term>Present study</term><term>Primer</term><term>Proc natl acad</term><term>Progesterone</term><term>Proliferative</term><term>Proliferative phase</term><term>Proliferative phases</term><term>Protein levels</term><term>Receptor</term><term>Reprod</term><term>Salamonsen</term><term>Santa cruz</term><term>Secretory</term><term>Secretory phase</term><term>Starvation media</term><term>Steroid</term><term>Steroid hormones</term><term>Transporter</term><term>Trpv6</term><term>Trpv6 mrna</term><term>Uterine</term><term>Uterine endometrium</term><term>Uterine trpv6</term><term>Uterus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Maintenance of calcium balance in the uterus is essential for many of its functions, including embryo implantation. The plasma membrane Ca2+‐pumping ATPase proteins are encoded by four genes designated PMCA1‐4, and PMCA1 is expressed in the uterus of rats during the estrous cycle. Although transient receptor potential cation channel subfamily V, member 6 (TRPV6), has been detected in the human placenta, pancreas and the prostate gland, expression patterns of uterine TRPV6 and PMCA1 and their potential roles in the human endometrium remain to be elucidated. In the present study, the expression patterns of TRPV6 and PMCA1 were examined to predict their potential roles in the human endometrium during the menstrual cycle. Human classified endometrial tissues (total n = 40) were separated into three groups according to menstrual cycle phase: menstrual, proliferative (early‐, mid‐, late), and secretory phase (early‐, mid‐, late). The expression of TRPV6 and PMCA1 mRNA and protein in the uterine endometrium during the menstrual cycle increased by 1.5‐ to 1.8‐fold at the proliferative phase (early‐, mid‐, and late‐) in comparison to the other phases. Estrogen treatment caused a significant increase in TRPV6 and PMCA1 mRNA expression. Immunohistochemical analysis of the distribution of TRPV6 and PMCA1 in the uterus revealed that both proteins are abundantly expressed in the cytoplasm of endometrial and glandular epithelial cells during menstrual phases. Taken together, these results suggest that uterine expression of TRPV6 and PMCA1 may be involved in human reproductive function. Mol. Reprod. Dev. 78:274–282, 2011. © 2011 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Yang, Hyun" sort="Yang, Hyun" uniqKey="Yang H" first="Hyun" last="Yang">Hyun Yang</name></noRegion><name sortKey="Choi, Kyung Hul" sort="Choi, Kyung Hul" uniqKey="Choi K" first="Kyung-Chul" last="Choi">Kyung-Chul Choi</name><name sortKey="Hyun, Sang Wan" sort="Hyun, Sang Wan" uniqKey="Hyun S" first="Sang-Hwan" last="Hyun">Sang-Hwan Hyun</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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